Method-development software for HPLC is usually quote-only and costs thousands. HPLC Assist Pro is a self-serve browser tool: add your compounds from PubChem, pick a column and see a predicted chromatogram. Auto-Optimize searches column, %B and pH for the best resolution. Includes retention calibration, pH/pKa scouting, a gradient optimizer, USP/Ph. Eur./JP system suitability, an ICH Q2(R2) validation planner and a printable method report. Built by a pharma analytical scientist.